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500 PROGRESS OF MEDICAL SCIENCE. 

and of certain long, slender bacilli frequently found by him in the stomach- 
contents in such cases. 

The first of these micro-organisms, sarcina, is usually described as a fre¬ 
quent ingredient of the gastric contents of carcinoma, but this Oppler 
denies. He has repeatedly introduced sarcina in pure culture into the 
stomach in carcinoma cases, and has always in advanced cases noticed its 
disappearance in the course of twenty-four hours. In two cases sarcina was 
found early in the disease while hydrochloric acid was still present, but sud¬ 
denly disappeared a few days later when hydrochloric acid ceased to be 
present and lactic acid began to be formed in considerable quantity. Coin¬ 
cident with the disappearance of sarcina was the appearance of long, slender 
bacilli, which had been absent as long as hydrochloric acid continued. 
These soon became very numerous, forming long chains and masses, almost 
filling the interstices between the fragments of undigested food. 

These observations are believed by Oppler to throw much light upon the 
processes occurring in the stomach in carcinoma. The ability of sarcina to 
develop in the presence of hydrochloric acid is well known. Its presence, 
then, during the early stages of the disease depends upon diminished motor 
activity of the stomach and consequent stagnation of its contents. In later 
stages, after the disappearance of hydrochloric acid and the establishment of 
the lactic-acid fermentation, the conditions are favorable to the development 
of the slender bacilli above referred to, which multiply rapidly and displace 
the sarcina. Thus, while sarcina may be present in the early stages of car¬ 
cinoma, it is invariably absent later in the disease. Since these changes 
depend for their production to a considerable degree upon the existence of 
diminished motor activity of the stomach, they are usually observed earlier 
in the course of the disease when it iB located in the pylorus than when its 
site is elsewhere in the gastric wall. 

Oppler would accordingly formulate the conditions in carcinoma of the 
stomach as follows: 

1. In cases of preserved motor activity (as, for example, in carcinoma of 
the curvatures or walls), hydrochloric acid is often absent, occasionally lactic- 
acid fermentation, particles of the tumor and bacilli are found; sarcina never. 

2. When the motor activity iB decidedly impaired hydrochloric acid may 
be either present or absent. 

a. "When present (as in the early stages of cancer of the pylorus) sarcina 
may be found, but never lactic-acid formation nor the slender bacilli. 

b. When hydrochloric acid is no longer present (as in the later stages of 
carcinoma of any portion of the stomach) lactic-acid formation is pro¬ 
nounced, and with it large numbers of the slender bacilli. There is a ten¬ 
dency to gas-formation. Sarcina is always absent. 

The conditions enumerated under 1 and 2, a , may pass into those of 2, b r 
as the disease progresses. 

Suppurative Pabotitis Caused by the Typhoid Bacillus. 

To the list of suppurative processes complicating typhoid fever, in which 
the typhoid bacillus alone has been found as the cause of the abscess-forma¬ 
tion, Janowbki (Cenlralblaii f. Bait. u. Parasiienhmde, 1895, xvii.. No. 22, 
785) has recently added a case of suppurative parotids. 



PATHOLOGY AND BACTERIOLOGY. 


501 


The patient, a young man, had been sick in the hospital seven weeks, dur¬ 
ing which time he had greatly emaciated; had had more or less fever, and 
toward the last had developed hemorrhagic nephritis and a tender enlarge¬ 
ment of the right parotid gland. The diagnosis of typhoid fever was first 
made on the autopsy-table, Peyer’s patches showing distinct appearances of 
recently healed ulceration. The right parotid gland was found to be infil¬ 
trated with pus, which was in places collected in small abscesses. Cultures 
from this pus developed only the typhoid bacillus, whose identity was care¬ 
fully proved by comparison with known cultures of the typhoid bacillus and 
of the bacillus coli communis. 

As has been repeatedly observed in connection with suppurative processes 
due to the bacillus typhosus, the progress of the complication was slow, so 
that the parotitis became fully developed only after the termination of the 
primary disease. 

Those cases of suppurative parotitis occurring in connection with typhoid 
fever, which have heretofore been examined bacteriologically, have been found 
to be due to the ordinary pyogenic bacteria, staphylococcus pyogenes aureus and 
streptococcus pyogenes, or to have contained one or the other of these germs in 
association with the typhoid bacillus. JanowBki’s is the first case in which 
the bacillus typhosus has been found alone. 

A Method op Quick Examination of Pathological Tissues poe 
Diagnostic Pubposes. 

It is very often desirable to be able to make a diagnosis of tumors and 
other pathological tissues at the time of operation or as soon thereafter as 
possible. Many difficulties have attended the employment of frozen sections 
prepared in the ordinary way, but by a slight modification of the procedure 
Cullen (Centralblatt AUg. Path. u.f. Path . Anat., 3895, vi., No. 11, 448) has 
been able to obtain well-stained and satisfactory sections in fifteen minutes. 

Frozen sections are first made, and are collected in normal salt-solution. 
They are then immersed for five minutes in a 50 per cent, solution of for¬ 
malin, for three minutes in 50 per cent, alcohol, for one minute in absolute 
alcohol; then washed in water and stained in hmmatoxylin and eosin after 
the ordinary method; are dehydrated, cleared, and mounted in balsam. 

The difficulty attending this method is that the blood is lost. By a some¬ 
what longer process this may be avoided. Small pieces of the tissue to be 
examined are immersed in a 10 per cent, solution of formalin for two hours. 
Frozen sections are then made, and are carried through 50 per cent, alcohol, 
absolute alcohol, water, hcematoxylin, etc., as before. 

These methods have yielded good results at the laboratory of the Johns 
Hopkins Hospital, and are well spoken of by Prof, von Kalhden, of Freiburg. 

Heredity and Tuberculosis. 

Kuthy (Pester Med.-Chirurg. Presse, 1894, No. 51) has attempted by a 
statistical study of 432 cases of tuberculosis to determine the influence of 
heredity in the etiology of that disease. In 23.8 per cent, of the cases one or 
both parents had the disease; in 11.5 per cent., the father; in 9.9 per cent., 
the mother, and in 2.4 per cent, both parents.